Genomic dissection of plant development and its impact on thousand grain weight in barley through nested association mapping

نویسندگان

  • Andreas Maurer
  • Vera Draba
  • Klaus Pillen
چکیده

Flowering time is a key agronomic trait that plays an important role in crop yield. There is growing interest in dissecting the developmental subphases of flowering to better understand and fine-tune plant development and maximize yield. To do this, we used the wild barley nested association mapping (NAM) population HEB-25, comprising 1420 BC1S3 lines, to map quantitative trait loci (QTLs) controlling five developmental traits, plant height, and thousand grain weight. Genome-wide association studies (GWAS) enabled us to locate a total of 89 QTLs that genetically regulate the seven investigated traits. Several exotic QTL alleles proved to be highly effective and potentially useful in barley breeding. For instance, thousand grain weight was increased by 4.5 g and flowering time was reduced by 9.3 days by substituting Barke elite QTL alleles for exotic QTL alleles at the denso/sdw1 and the Ppd-H1 loci, respectively. We showed that the exotic allele at the semi-dwarf locus denso/sdw1 can be used to increase grain weight since it uncouples the negative correlation between shoot elongation and the ripening phase. Our study demonstrates that nested association mapping of HEB-25 can help unravel the genetic regulation of plant development and yield formation in barley. Moreover, since we detected numerous useful exotic QTL alleles in HEB-25, we conclude that the introgression of these wild barley alleles into the elite barley gene pool may enable developmental phases to be specifically fine-tuned in order to maximize thousand grain weight and, potentially, yield in the long term.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Identification of genomic loci controlling phenologic and morphologic traits in barley (Hordeum vulgare L.) genotypes using association analysis

Association mapping is a technique with high resolution for QTL mapping based on linkage disequilibrium and has shown more promising for describing genetically complex traits. In addition, it is a powerful tool for describing complex agronomic traits and identifying alleles that can contribute to enhance the desired traits. In this study, whole genome association mapping was used in a set of 14...

متن کامل

Determination of QTLs Associated with Agronomic and Physiological Traits under Normal and Salinity Conditions in Barley

mapping the QTLs of agronomic and physiological traits, 149 double haploid (DH) lines from a cross between an Australian cultivar, Clipper (salt susceptible), and an Algerian landrace, Sahara3771 (salt tolerant), were evaluated under natural saline (Yazd Station, ECsoil=10-12.8 ds/m and ECwater= 9-10 ds/m) and normal (Karaj Station, ECsoil and ECwater ~2-2.5 ds/m) environments. There were remar...

متن کامل

Association Analysis for Important Quantitative and Morphological Traits in Cultivars and Advanced Lines of Soybean (Glycine max (L.)) using Microsatellite Markers

IExtended Abstract Introduction and Objective: The economic value of a genotype depends on its various traits and therefore the accurate knowledge of genetic behavior and identification of genomic locus involved in controlling these traits can help the breeder to improve genotypes. Material and Methods: In this study, the relationship between microsatellite markers with some important agrono...

متن کامل

Evaluation and Characterization of Barley Inbred Lines in Temperate Regions of Country

Extended Abstract Introductionand Objective: Barley (Hordeum vulgare L.) with a cultivated area of ​​nearly one and a half million hectares and with a production of about three million tons per year after wheat is the main crop in the country. The main objectives of this study were to (i) examine the variation within the barley national germplasm, (ii) compare the introduced germplasm with the...

متن کامل

Improving barley performance by proper foliar applied salicylic-acid under saline conditions

Despite general effect of salicylic-acid (SA) in improving plant growth andproductivity in saline conditions, there have not been unanimity about the bestconcentration. In this 2-yr field study the effect of different SA concentrations(0, 0.5, 1.0, 1.5 and 2.0 mM) was examined on growth, grain yield and yieldcomponents of barley under two non-saline (2 dS m-1) and saline (12 dS m-1)conditions. ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره 67  شماره 

صفحات  -

تاریخ انتشار 2016